Identified miRNAs LM(nt) miRNA sequences Input sequence Strand Location LP(nt) MFEs(DG) MFEI
lme-miR156a20UGACAGAAGAGAGUGAGCACscaffold675+/+5091-44.31.05
lme-miR156a-3p22GCUCACUGCUCUUUCUGUCAGAscaffold568+/+30128-47.90.87
lme-miR156b-3p23UGCUCACCUCUCUUUCUGUCAGUscaffold236+/+30101-45.50.97
lme-miR156c-3p22GCUCACUGCUCUAUCUGUCAGAscaffold675+/+3086-43.81.07
lme-miR157a21UUGACAGAAGAUAGAGAGCACscaffold344+/+50131-49.41.01
lme-miR157a-3p21GCUCUCUAGCCUUCUGUCAUCscaffold78+/+3086-44.41.35
lme-miR157c-3p21GCUCUCUAUACUUCUGUCACCscaffold344+/+30151-54.90.96
lme-miR159c21UUUGGAUUGAAGGGAGCUCCUscaffold613+/-30201-66.60.89
lme-miR160a21UGCCUGGCUCCCUGUAUGCCAscaffold249+/+5081-45.71.04
lme-miR160a-3p21GCGUAUGAGGAGCCAUGCAUAscaffold249+/+3096-48.70.99
lme-miR160c-3p21CGUACAAGGAGUCAAGCAUGAscaffold468+/-3090-40.31.03
lme-miR161.221UCAAUGCAUUGAAAGUGACUAscaffold216+/+50102-40.20.91
lme-miR162a22UGGAGGCAGCGGUUCAUCGAUCscaffold1363+/+50116-37.20.77
lme-miR162a-3p21UCGAUAAACCUCUGCAUCCAGscaffold1363+/+30131-44.30.76
lme-miR164a21UGGAGAAGCAGGGCACGUGCAscaffold668+/-50126-45.20.92
lme-miR164c21UGGAGAAGCAGGGCACGUGCGscaffold369+/+5083-38.90.97
lme-miR165a22UGGAGGCAGCGGUUCAUCGAUCscaffold1363+/+50117-41.90.86
lme-miR165a-3p21UCGGACCAGGCUUCAUCCCCCscaffold830+/+30125-45.41.05
lme-miR166a21GGACUGUUGUCUGGCUCGAGGscaffold316+/+50151-64.31.07
lme-miR166a-3p21UCGGACCAGGCUUCAUUCCCCscaffold774+/+3092-32.30.73
lme-miR167a21UGAAGCUGCCAGCAUGAUCUAscaffold785+/+5097-50.71.24
lme-miR167c21UAAGCUGCCAGCAUGAUCUUGscaffold695+/-50146-681.26
lme-miR168a21UCGCUUGGUGCAGGUCGGGAAscaffold468+/-50101-46.60.91
lme-miR168a-3p21CCCGCCUUGCAUCAACUGAAUscaffold369+/+30101-47.40.89
lme-miR169a21CAGCCAAGGAUGACUUGCCGAscaffold191+/+50227-67.81.09
lme-miR169b21CAGCCAAGGAUGACUUGCCGGscaffold872+/+50216-59.50.94
lme-miR169d21UGAGCCAAGGAUGACUUGCCGscaffold792+/+50121-45.90.9
lme-miR169h21UAGCCAAGGAUGACUUGCCUGscaffold1032+/+50131-43.30.92
lme-miR169b-3p22GGCAAGUUGUCCUUCGGCUACAscaffold467+/+30112-58.61.43
lme-miRmiR17021UAUUGGCCUGGUUCACUCAGAscaffold1132+/+5083-33.60.96
lme-miR170-3p21UGAUUGAGCCGUGUCAAUAUCscaffold1132+/-3081-27.40.78
lme-miR171b21AGAUAUUAGUGCGGUUCAAUCscaffold490+/-5091-36.80.99
lme-miR171a-3p21UGAUUGAGCCGCGCCAAUAUCscaffold299+/+3085-33.60.93
lme-miR171b-3p21UUGAGCCGUGCCAAUAUCACGscaffold433+/+3096-40.31.01
lme-miR172a21AGAAUCUUGAUGAUGCUGCAUscaffold981+/+30105-35.91.12
lme-miR172c21AGAAUCUUGAUGAUGCUGCAGscaffold997+/+30106-40.71.13
lme-miR319a21UUGGACUGAAGGGAGCUCCCUscaffold1047+/+30181-76.21.14
lme-miR319c21UUGGACUGAAGGGAGCUCCUUscaffold488+/-30173-69.60.98
lme-miR390a21AAGCUCAGGAGGGAUAGCGCCscaffold237+/+5091-43.41.11
lme-miR39121UUCGCAGGAGAGAUAGCGCCAscaffold432+/+5082-34.80.87
lme-miR391-3p21ACGGUAUCUCUCCUACGUAGCscaffold432+/+3087-36.30.84
lme-miR393a22UCCAAAGGGAUCGCAUUGAUCCscaffold997+/+50156-53.91
lme-miR393b-3p21AUCAUGCGAUCUCUUUGGAUUscaffold997+/+30141-50.91.04
lme-miR394a20UUGGCAUUCUGUCCACCUCCscaffold420+/+50126-470.85
lme-miR394b-3p21AGGUGGGCAUACUGCCAAUAGscaffold356+/+30111-48.71.08
lme-miR395a21CUGAAGUGUUUGGGGGAACUCscaffold904+/-3092-44.31.1
lme-miR395b21CUGAAGUGUUUGGGGGGACUCscaffold904+/+30106-35.91
lme-miR396a21UUCCACAGCUUUCUUGAACUGscaffold339+/-50106-34.50.88
lme-miR396b21UUCCACAGCUUUCUUGAACUUscaffold127+/+50132-43.71.15
lme-miR396a-3p21GUUCAAUAAAGCUGUGGGAAGscaffold306+/+30117-41.30.96
lme-miR396b-3p21GCUCAAGAAAGCUGUGGGAAAscaffold127+/+30156-55.51.13
lme-miR397a21UCAUUGAGUGCAGCGUUGAUGscaffold299+/-5096-26.80.96
lme-miR398a-3p21UGUGUUCUCAGGUCACCCCUUscaffold302+/+30101-42.91.13
lme-miR398b-3p21UGUGUUCUCAGGUCACCCCUGscaffold534+/-30115-42.80.82
lme-miR399a21UGCCAAAGGAGAUUUGCCCUGscaffold904+/-30126-41.60.97
lme-miR399b21UGCCAAAGGAGAGUUGCCCUGscaffold971+/-30146-51.81.01
lme-miR399c-3p21UGCCAAAGGAGAGUUGCCCUGscaffold971+/-30141-51.21.04
lme-miR40821ACAGGGAACAAGCAGAGCAUGscaffold613+/+5096-39.50.9
lme-miR408-3p21AUGCACUGCCUCUUCCCUGGCscaffold613+/-30101-39.60.84
lme-miR82822UCUUGCUUAAAUGAGUAUUCCAscaffold236+/+50101-35.81.02
lme-miR2111a21UAAUCUGCAUCCUGAGGUUUAscaffold668+/+50101-38.31.18
lme-miR2111b-3p21AUCCUCGGGAUACAGUUUACCscaffold602+/+3076-29.91.11



Potential targets of identified maca miRNAs


miRNATargeted proteins (number)
156/157 Squamosa promoter-binding-like protein (24)
159 MYB (4)
159Pectin acetylesterase (1)
159Pyruvate orthophosphate dikinase (5)
160 Auxin response factor (4)
162 Cytochrome P450-like protein (1)
164 CUP-SHAPED COTYLEDON/CUC (3)
164 NAM protein-like (6)
165 HD-Zip protein (2)
166 HD-Zip protein (2)
168 Glutathione transferase (1)
171 Scarecrow-like protein (2)
171 Protein kinase (2)
172 APETALA 2 (8)
172MYB (2)
319 MYB (3)
390 Protein kinase (1)
390Glutamate dehydrogenase (1)
391 Protein kinase (2)
393 WRKY transcription factor (1)
394 F-box only protein (1)
394Lipase-like protein (1)
394Loricrin-like protein (1)
395 ATP sulfurylase (9)
395Transcription factor bZIP (2)
397 Laccase precursor (3)
399 F-box protein (1)
399Cytochrome P450-like protein (1)
408 Ethylene-responsive transcription factor (1)
828 Kinesin 2 (2)
2111 Flavanone 3-hydroxylase-like protein (2)
Source: Identification and characterization of microRNAs and their targets in high-altitude stress-adaptive plant maca (Lepidium meyenii Walp)